My server is as
server { server_name .domain.com;
listen 80;
root /home/domain.com;
index index.html index.php;
location ~ \.php$ {
fastcgi_pass 127.0.0.1:9000;
fastcgi_index index.php;
fastcgi_param SCRIPT_FILENAME $document_root$fastcgi_script_name;
include /opt/nginx/conf/fastcgi_params;
}
}

You mean to add another location as
location ~ \.py$ {
fastcgi_pass 127.0.0.1:9000;
fastcgi_index index.py;
fastcgi_param SCRIPT_FILENAME $document_root$fastcgi_script_name;
include /opt/nginx/conf/fastcgi_params;
}